Investigating monocyte chemotactic protein–1 (MCP–1) and chemokine receptor–2 (CCR2) variants of C-C motif chemokine family in patients with solid tumors

Solid tumors are named according to their cell origins and account for most of the cancer cases. Solid tumors can be grouped as malign and benign tumors. In the present study, lung cancer, colorectal cancer, breast cancer, ovarian cancer and throid cancer cases were included to our study pool of solid tumors and 28 lung cancer (4 female and 24 male), 34 colorectal cancer (12 female and 22 male), 23 thyroid cancer (20 female and 3 male), 27 breast cancer (25 female and 2 male) and 30 ovarian cancer cases were analyzed. We analyzed 66 healthy controls (27 female and 39 male). CCR2 V64I variants were determined via enzyme restriction with BsaB1 endonuclease enzyme and genotypes of MCP-1 A2518G variants were determined via enzyme restriction with PvuII endonuclease enzyme. Both enzymes were selected as not to restrict the wild type PCR products. Statistical analysis were performed using IBM SPSS v.20 software. According to present study, the relation between CCR2 V64I variants and colorectal cancer and lung cancer was statistically significant (p<0,0001). In addition, the relation between MCP-1 A2518G AG and AA genotypes and breast cancer were also statistically significant (AG genotype χ2=5,082; p<0,024, OR:2,843, %95CI: 1,129-7,160 and AA genotype χ2=5,687; p<0,017, OR:0,319, %95CI: 0,122-0,833). Present study is the first study investigating the relation between CCR2 V64I variant, MCP-1 A2518 variant and colorectal cancer, ovarian cancer and thyroid cancer.