Süt ve balda sülfonamidlerin kromatografik tayini

The sulfonamides are collected in animal tissues and organs and then they accumulate in human kidney and hepatic by following the chain of consumption. For these reasons, determination of sulfonamides becomes essential because of inhibiting folic acid synthesis and causing permanent damage.In this study, preconcentration process was performed for food products such as milk and honey with a surfactant Triton X-114 and high pressure liquid chromatography diode array detector (HPLC-DAD) was used to determine sulfadiazine (SDA), sulphamethoxazole (SMZ). Cloud point extraction (CPE) which is simple, fast, new and economic and uses surfactants fundamentally as an alternative instead of organic solvents, concerns green chemistry concept was performed for the extraction of sulfonamides. Quantitative analysis of extracts was performed in methanol/water (22:78, v/v) isocratic elution at 1.0 mL/min flow rate with 35 degrees Celsius at 270 nm after pH, salt & surfactant concentration, incubation time and temperature optimizations were completed.Detection and quantitation limits of the method were found as 5.68 and 20.09 ppb and 6.06 and 21.86 ppb for SDA and SMZ, respectively, the linear range of quantitation for analytes was approximately 0.025 - 2.000 ppm, the percentages of average recovery as 65.2% and 99.4% and the relative standard deviation percentage as 0.51% and 2.17% were found for SDA and SMZ, respectively.The results of the amount of sulfonamides in two kinds of milk and ten kinds of honey were determined as in the range of 0.062 - 0.104 ppm and 0.017 - 0.643 ppm (SDA), 0.018 - 0.038 ppm and 0.006 - 0.162 ppm (SMZ), respectively.