Genotyping of E.coli strains isolated from fresh cheese

The aim of this study was to evaluate phylogenetic and ecological diversity of E.coli strains isolated from raw cheese samples produced in Aydın province, by genotyping with RAPD-PCR technique. In this research, the fresh cheese materials produced at different dairy houses were obtained from dairy markets, local markets, district markets in and near Aydın province and they were investigated for colony forming unit of coliform bacteria and E.coli presence. 250 gr pieces of 100 fresh cheese samples that were obtained from different markets were taken in sterile bags and were brought to Adnan Menderes University. Veterinary Faculty, Food Hygiene and Technology Department laboratories in cold chain. After the enrichment and selective media processes, the isolated bacteria were identified by bio-chemical tests and PCR. After then, isolates were genotyped by using RAPD-PCR. As a result, in our study, coliform bacteria found in 77 fresh cheese materials at the average level of 4,83 log cfu/g., 44 E.coli isolates were identified in 22 of the 77 coliform contaminated samples.. The obtained 44 isolates were also verified as E.coli by PCR. 22 different genotypes were determined by genotyping of these isolates using RAPD-PCR.