Studies on bacterial enzymes isolated from thermal resources

In this study, bacterial isolates were isolated from the Bingöl Ilicalar landfill samples due to the importance of bacterial enzymes isolated from hot water sources in the industrial area. It is aimed to investigate the production of industrially important enzymes by these isolated bacteria and to work on these enzymes. In this study, 2 bacteria were isolated in isolated water and soil specimens. Various biochemical tests, morphological and physiological analyzes of these bacteria were performed. It was determined that the isolated 4NK bacteria were rod-shaped gram positive and 5NK bacteria were gram positive and rod-shaped. The optimum pH value for these two isolated bacteria was determined to be 6. Optimum reproductive temperatures were 40 and 45 oC, respectively. Once the optimum conditions of the isolated bacteria have been determined, attempts have been made to determine the optimums of the enzymes they produce. Accordingly, it has been determined that the optimal time for production of protease and amylase enzymes of 4NK bacteria is 24th and 15th hours, respectively. The optimum pHs of protease and amylase enzymes were 8 and 4-11, respectively; optimum temperatures were found to be 40 and 60 oC, respectively. The optimal time for production of protease and amylase enzymes of 5NK bacteria was determined to be 24th and 12th hours, respectively. The optimum pHs of protease and amylase enzymes of this bacterial variant were 8 and 4-11; the optimum temperatures were found to be 50 oC. . 16S rRNA sequence analysis of isolated 4NK and 5NK bacteria was performed and it was determined which species and subspecies are phylogenetically close to each other. It was determined that the resultant 4NK bacteria were close to Bacillus subtilis and 5NK bacteria were phylogenetically close to Bacillus paralicheniformis. Keywords: Amylase, protease, hot water source, enzyme, Bacillus