Microbial decolorization of commonly used dyestuffs in usak textile industry

They contain large amounts of organic and inorganic pollutants in textile wastewater. Wastewater discharged to nature damages many living microorganisms and negatively affects photosynthetic activity by reducing the light transmittance in the water. In addition, the accumulation of dyes in some aquatic organisms brings with it the risk of toxic and carcinogenic products. The color removal of azo dyes (Acid Blue, Acid Red) of Bacillus isolates determined for this purpose was investigated. The textile waste collected from the Uşak organized industrial zone was isolated from organized waste and ceramic waste, and 8 Bacillus sp were obtained. Microscopic, macroscopic structures of the isolates were examined. It was determined that all isolates had Bacillus, biochemical characterization (Catalase, starch hydrolysis, NaCl, VP and citrate test) SA1, SA2, TA4, TA5 species were determined. The optimum growing temperature was determined to be 37-40 ˚C. As a result of 16S rRNA sequences, SA1 (Bacillus mycoides), SA2 (Bacillus licheniformis), SA3 (Bacillus thuringiensis), TA4 (Bacillus licheniformis), TA6 (Bacillus flexus), OA7 (Bacillus proteolyticus), OA8 (Bacillus proteolyticus) determined. It was determined that Bacillus sp. adsorbed more Acid Red and Acid Blue dyes when incubated at 37˚C in the LB medium for 24-220 hours in a shaking oven (1600rpm). TA4 isolate was demonstrated maximum Acid Blue decolorization (47,08%) and OA7 isolate was demonstrated maximum Acid Red decolorization (69,74%). In FTIR analysis, it was determined that it adsorbed Acid Blue on bacteria carbonyl functional and Acid Red aromatic bond groups.