Histomorphometric analysis of the effects of grape seed extract (Vitis vinifera) and low-level laser therapy (LLLT) on fracture heali̇ng

The aim of this study was to research the effect of Grape Seed Extract (GSE) and Low Laser Level Therapy (LLLT) on the healing of experimentally fractured mandible and their effect on the oxidant and antioxidant system. Our study included 48 adult male Wistar Albino rats. A vertical fracture line were performed in right mandibule passed between the molar teeth of all experimental animals, then the fracture line were fixed internally with four-holed microplate and four micro screw. Subjects were divided randomly to four groups (Control, GSE, LLLT, GSE+LLLT) so that each group included 12 animals, then each group were subdivided to two groups according to the 7th and 21st day of the experiment and thus 8 (n=6) groups were formed. GSE was given to the subjects as 300 mg/kg/day until the day they were sacrificed. LLLT was applied to the 7th day groups for 7 days and to the 21st day for 14 days with 48 hour intervals on 2 different points along the fracture line at a dose of 23 J/cm2. According to the biochemical investigations made in both 7.th and 21st day, only TAS values were found to be statistically significant difference. The histological examinations showed that the fracture healing in GSE+LLLT and GSE groups were found to be significantly better than the control group. Only the LLLT group was not able to show sufficient efficacy for healing, however when it used combined with the GSE, the highest level of ossification was detected. Keywords: Low-level Laser therapy, Fracture healing, Oxidative stress, Free radicals, Grape seed extract