Virulence factors and antibiotic resistance of Klebsiella pneumoniae isolated urinary tract infection (UTI) patients suffered from diabetes mellitus type two in Iraq

Klebsiella pneumoniae (K. pneumoniae) was found in 150 out of 220 urine samples when diagnosed by the Vitek 2 Compact system. The urinary infection rate was higher in females (56.7%) than males (43.3%). While there was a positive correlation between age and HbA1C, there was a negative correlation between HbA1C and urea. A negative correlation was found between creatinine and UTI period, resulting in significant differences (P<0.05). Through Vitek 2 compact used to know its antibiotic resistance, K. pneumoniae appeared a high resistance to Cefazolin (88%), Amoxicillin and Clavulanic Acid (86%), Cefuroxime (80%), Cefuroxime Axetil (70%), Ceftazidime (68%) and Piperacillin / Tazobactam (62%). The virulence genes detected by PCR were the clbA gene in 24 (16%) isolates and rmpA in 54 (36%) isolates. The sequencing results were observed for the rmpA gene in one isolate that had a compatibility of 99% with the reference strain Acc.No: CP035540.1 and regarded as one type of substitution (transversion). Other isolates had a compatibility of 100% with reference strain Acc.No: LR890726.1, and the sequencing results of the clbA gene in isolates had a compatibility of 100% with the reference strain Acc.No: LT603726.1.