Determination and characterization of extracellular hydrolytic enzyme capacities of bacteria isolated from Zerene hot spring (Hakkari)

The material of this research; Water and sediment samples collected in Zerene Thermal Spring, located within the borders of Sarıtaş Village of Hakkari Province, were formed. The ability of 4 bacteria isolated from water and sediment samples to produce amylase, lipase, cellulase, protease, xylanase, L-asparaginase and L-glutaminase was examined. All isolates were found to be negative in terms of lipase, xylanase, cellulase and L-glutaminase enzymes. The amylase enzyme MN001 and MN010, protease enzyme MN003, MN004 and MN010 showed the ability to produce isolates. It was determined that all isolates produced L-asparaginase enzyme. Isolates were identified according to their morphological, physiological, biochemical characteristics and 16S rRNA sequence analysis. After the genomic DNA of the isolates was isolated, PCR amplification of the 16S rRNA gene region was performed with the universal primers 27F and 1492R. A Maximum Likelihood phylogenetic tree was constructed to determine the genetic distance between species. It was observed that the isolates clustered with strong homology in the phylogenetic tree and their genetic positions were revealed. As a result of the comparative 16S rRNA gene sequence analysis, it was determined that the isolate MN001 was 99.59% similar to Bacillus licheniformis, MN003 was 100% similar to Bacillus oceanisediminis, MN004 was 100% similar to Bacillus subtilis, and MN010 was 100% similar to Bacillus halotolerans.