Investigation of anti proliferative and anti-oxidant potential of Anemone blanda plant extract in BEAS-2B and A549 cell lines in vitro

Cancer continues to be one of the leading causes of morbidity and mortality worldwide. Cancer is the second leading cause of death after cardiovascular diseases, excluding noncommunicable diseases. Today, research on various herbal products is increasingly continuing to develop new drugs against diseases such as cancer. In recent years, promising anti-cancer efficacy of various herbal products against different cancers has been demonstrated. In this context, in the thesis study, it was aimed to investigate the anticancer potentials of methanolic extracts obtained from Anemone blanda plant. For this purpose, the human healthy bronchial epithelial cell line Beas-2B and the human carcinoma cell line A549 cells were used. XTT test and Clonogenic test were used to determine cytotoxicity, Komet method to determine DNA damages and ROS test to determine their effects on intracellular reactive oxygen level. As a result of the XTT test, the IC50 values in Beas-2B and A549 cell lines were determined as 21.90 µg/mL and 21.25 µg/mL, respectively. In the clonogenic test, these values were determined as 24.57 µg/mL and 27.62 µg/mL for Beas-2B and A549 cell lines, respectively. In the Comet test, it was used in XTT and Clonogenic tests, in addition to the doses, it was added in combined doses with H2O2. Tail length, tail % DNA and olive tail moment in Beas-2B and A549 cell lines were calculated, and it was determined that there were statistically significant increases in DNA strand breaks compared to the control group, while it was statistically decreased at doses with H2O2. While the same doses as in the comet test were used in the ROS test, there was no statistically significant change in the intra-ROS level for Beas-2B in the results. In combined doses, it was observed that the mean intracellular ROS level of Anemone extract was statistically significantly reduced from 40 µg/mL dose, while it statistically decreased the ROS level for A549, while it decreased the intracellular ROS level statistically at combined doses, but 100µg/ml+1000 µM H2O2' It was observed that the extract at a combined dose of 1,0 increased the effect of H2O2 and increased the intracellular ROS level statistically significantly. Keywords: Anemone blanda, Lung cancer, anti-cancer, A549, Beas-2B, Clonongenic test, Comet test, ROS test, XTT test