Adverse effects of anti-rat vasculer enrothelial growth faktor on kidney function and morphology, can be prevented by lisinopril ?

SUMMARYAim: By creating an experimental rat model,that we can observe the adverse affects of Anti-rat VEGF on the kidneys of the rats ,evaluating the effects on the kidney as dose and time-dependent and observing Lisinopril,which was a concomitant angiotensin-converting enzyme (ACE) inhibitor,to what degree prevent these effectsMethod: With the avarage weight of 160 gr,72, 3 months female wistar albino rats were taken to test.Rats ,firstly as 1,2,and 3, are seperated equally 3 main groups according to their sakrification days (respectively 1st ,3rd and 14th days). 24 rats within each main group (respectively, A, B, C and D) were divided into four sub-groups which consist of 6 rats. A sub- group rats were given IV 3.25 picomol anti ?rat VEGF,B sub-group rats were given İV 3.25 picomol anti-rat VEGF, C sub group rats were given İV 3.25 picomol anti-rat VEGF and D sub-group rats were given İV 3.25 picomol anti-rat VEGF. 75 mg/L Lisinopril was added into drinking waters of sub-groups C and D in 3 main groups.Results: In the first group, daily protein excretion after drug,increased in all sub-groups except sub-group B. Creatinine clearance decreased in all sub-groups.In the second group daily protein excretion decreased in all sub-groups except sub-group B,and the creatinine clearance decreased in all sub-groups.In the third group, daily protein excretion increased in all sub-groups except for sub-group A, creatinine clearance was significantly increased in all subgroups except sub-group B .In A and B sub group rats that were sacrificed in the first day,there were more glomerulosclerosis,focal necrosis in glomerular ,expansion in the range of bowman capsules, degeneration of tubular epithelium, necrosis in tubule epithelium, congestion and vascular wall thickening than other groups. In rats that were sacrificed in third day, only the degree of tubule epithelial degeneration was significiantly more in A and B sub-groups.in A sub-grops ,expansion in the range of bowman capsules,degeneration of tubuler epithelium and tubule dilatation were decreased and focal necrosis in glomerule and necrosis in tubule epithelium were recovered. In the rats that were sacrificed in the 14th day,there weren?t any difference to constitute statistical significance in terms of pathological findings among all the sub-groups. Pathological findings observed in first-day group, was significantly decreased at the level of all sub-groups in the 14th day group.Result: By using anti-rat vegf , in an experimental rat model, that we observe the adverse effects on its kidney, anti-rat vegf caused proteinuria, even at very low levels.anti-rat vegf application dose doesn?t related to having the level of proteinuria in the early period. pathological findings observed in the first day, significantly reduced the level of all sub-groups in the fourteenth day, to do with anti-VEGF in a rat experimental model is not appropriate for the 14-day period was concluded .Keywords: Anti-rat VEGF, kidney, lisinoprilContact address: University of Adnan Menderes ,Medical Faculty, Department of Internal Medicine, Aydın. Türkiye. drozlemayvaz@yahoo.com.tr.