Proteome profiling of Drechslera teres f. maculata, the causal agent of net blotch disease of barley

Barley is the second most important crop after wheat, in Turkey. According to FAO statistics Turkey is among the top 8 countries. Various biotic and abiotic stresses, limited use of certified seeds and disease resistant cultivars, limited knowledge about the seeds by farmers, decrease of land suitable for barley cropping due to erosion and drought affect the barley production and quality. Fungal diseases are the most important diseases affecting barley yield. Net blotch disease caused by Drechslerea teres is an important disease of barley. The fungus has two biotypes. Drechslera teres f. teres forms net type symptoms and Drechslera teres f. maculata forms spot type symptoms. Both forms are present in Turkey, however, spot form is more common. In this study, we aimed to obtain proteome profile of Drechslera teres f. maculata isolate high virulence GPS 79. Jel based proteomics approach was applied. Total protein extraction was performed by phenol-SDS method. Extracted protein was separated using 2-DE. 932 protein spots common within the biological replicates treated with trypsin. Tryptic peptides belonging to 108 spots were analyzed with nano-LC-MS/MS. As a result of 158 proteins were identified against 108 spots four spots did not give any hit the protein in database, 94% of identified proteins are hypothetical. 61% of these are predicted as cytosololic proteins. For we predicted 10 unique proteins belonging to 12 spots are predicted as extracellular protein and they have signal peptidies so these proteins are effector can the date proteins that are responsible from virulency of pathogen.