Serum and ascitic fluid total oxidative status and total anti-oxidative status in differential diagnosis of ascites.

The differential diagnosis of ascitic fluid is an important ıssue. It could be diffuculties in clinical practice. Oxidative stress has a role in the pathogenesis of several diseases causing to ascitic formation. In this study, we evaluate total oxidative status and total anti-oxidative status levels in ascitic fluid and in the serum. It is aimed to detect the oxidative status in cases with ascitic fluid secondary to different ethiologies and the oxidative reaction in benign and malign ascites. We investigated the effect of oxidative stress in cirrhotic ascites and in spontaneus ascit infections the total oxidative status and total anti-oxidative status levels in ascitic fluid and in the serum100 patients with ascitic fluid are classified in four groups. The groups are defined as following; 34 patients with cirrhotic ascites, 33 patients with spontaneus ascit infections, 28 patients with malign ascites and 5 patients tuberculous peritonitis. The ethilologies were HBV (n:28), HCV (n:21), criptogenic (n:14), alcholic liver cirrhosis (n:4). 28 Patients with malign ascites and 5 patients with tuberculous peritonitis are included in the studyThe serum TOS levels in malign group is statistically higher than another groups (p=0.001). In group with liver cirrhosis secondary to alchol the serum TOS levels are found statistically higher than groups with tuberculous peritonitis (p=0.027) and with HCV (p=0.045). The serum TOS levels in malign group are statistically higher than in benign group (p=0.048). The serum TAS levels in cirrhotic patients are statistically higher than in another group (p=0.041When the ascit TOS levels are compared all groups, there is no significance between the groups( p>0.05). In %80 of the patients, the ascit TOS levels are too low to detect. The ascit TAS levels are statistically not different between groups (p>0.05In conclusion, when the TOS and TAS levels are evaluated, the balance between oxidan- and antioxidan status in patients with malign ascites and with liver cirrhosis secondary to alchol is disturbed. These patients were exposed, to oxidative stress more than another groups. The fact that the TOS levels in cystemic circulation couldn't be measured in ascitic fluid, implies us the oxitadive stress is not produced locally in peritoneal cavity. However, there is need for further studies in differential diagnosis of ascite to assess the effect of locally produced oxidative stressKey words: Ascitic Fluid, Total Oxidative Status, Total Anti-oxidative Status