Biochemical alterations in rainbow trout (Oncorhynchus mykiss) after acute exposure to sildenafil

Sildenafil (Viagra) is a drug used to treat erectile dysfunction in men. Aquatic organisms expose to this chemical due to the increased usage of it. However, its effect has not been well studied in fish. The aim of this study was to examine the effect of 2 different doses of sildenafil on biochemical analysis including cytochrome P450 (CYP) and antioxidant enzyme activities in rainbow trout (Oncorhynchus mykiss). The animals were treated with sildenafil for 96 hours. At the end of this period, fish were killed and liver tissues were taken. The impact of sildenafil was measured in microsomes and cytosols of liver tissues. The results of this study showed that sildenafil had no acute effect on CYP1A-associated ethoxyresorufin O-deethylase, CYP3A-associated erythromycin N-demethylase, glutathione S-transferase, catalase and glutathione reductase activities. Sildenafil did not significantly alter cytochrome P450 and antioxidant enzyme activities in rainbow trout at these doses and within this administration period.