Comparision of two different methods for the determination of residues of tetracycline antibiotics in beef and chicken meat

In this study, it is aimed to determine the presence of antibiotics in chickens and calves, which are important for the nutrition due to the protein, fat, minerals and vitamins in the composition of the meat products. By evaluating the factors such as the amount of antibiotic residues, usage areas of antibiotics and frequency, it was tried to contribute to the work done in terms of food safety and community health. Even if there is a small amount of meat and meat products in their diet, the antibiotics may accumulate in the body, resulting in resistance to antibiotics in some bacterial strains, which may lead to a reduction in the effectiveness of antibiotics in human treatment. For the study, 20 poultry meat, 20 pcs of calf meat, which were sold outdoors in various sterile conditions, were collected from various butcheries in Samsun province and delivered to the laboratory in the cold chain. It was stored at -20 ºC until the day of operation. 12 of the beef samples taken from the breast were collected from a slaughter house on different days, 5 of them were butcher, and 3 of them were collected from the meat department of the big markets. Firstly, the antibiotic residues in the samples were qualitatively detected with the MeRA Test. MeRA test; Is a microbiological test containing Geobacillus stearothermophilus spores for the detection of antimicrobial substance residues. Tetracycline levels were quantitatively analysed by elisa (R-Biopharm) for the samples detected positively as a result of this test. Of the 20 red meat samples studied by the MeRA test, 10 were used; Of the 20 chickens, 19 were positive. That is, of the 40 meat samples studied in total, 29 were found above the acceptable limits. Positive samples were then examined for tetracycline levels by elisa. According to this test, tetracycline was found above the acceptable limits of 4 red meats and 17 chicken meat. Our study is a regional study, with more samples and samples in different regions should be expanded. However, it can be said that it is necessary to ask about the safety of the products that are sold outdoors.