Determination of antibiotic residues in egg samples by elisa

Antibiotics are drugs used in the treatment of infectious diseases. The most basic reason for not using in some foods is to prevent passing on to microorganisms found in the normal flora of animals .Microorganisms such as Salmonella and Campylobacter can be found in the gastrointestinal system, feathers and skin of poultry.Since the microorganisms are likely to be found in chickens,especially egg shells,some tetracyclines and similar antibiotics are given via drinking water. Because of unconscious uses of antibiotics,residues of antibiotics can be occur over the limits from time to time. In our study antibiotic level in eggs with highly day consuption were investigated. Organic and normal egg samples were collected and levels of chloramphenicol and tetracyclines were determined by ELISA method in the laboratory.In all four samples , residues of tetracycline were not found .These four samples are not packaged, but sold outdoors.In other not packed egg samples, it is determined as 0.4-70.8 ppb,and it is in the limits of maximum residue.But,for packed egg samples it was determined between 0,01-3,4 ppb. In 36 samples, none of the chloramphenicol residues were found,while the residues are detected in the four open samples. However, because of the residues of tetracycline are also over the limit, it is thougt to be cross react and can not be ascertained without being confirmed by HPLC management.It is pleasing that the level of the residues on the egg samples is low ,it is a fact that that is necessary to increase the number of studies by enlarging the sample.