Effect of zinc oxide nanoparticles use as alternative treatment for Pseudomonas aeruginosa

The current study included collection of 150 samples from Burns and Wounds during the period from October 2021 to January 2022. Patients with different ages ranging from 16-70 years from both genders were choosing from different hospitals in Baqubah city/Diyala Province, Iraq included Baqubah Teaching Hospital and Al-Batool Teaching Hospital. After culturing the specimens on general culture media, 121 of it were positive growth for bacteria. Pseudomonas agar, microscopic investigation, biochemical checks and VITEK 2 Compact were used to isolate Pseudomonas aeruginosa which consist 25 (16.6 %), 16 isolates were from Burns and 9 isolates were from wounds. The antibiogram of the isolates was tested against 10 antibiotics which were selected according to (CLSI 2021). The result showed that Cefepime and polymyxin B were the most resisted by the bacteria (100%), while Ciprofloxacin and Norfloxacin were the greatest in effect antibiotics in contrast to P. Aeruginosa (12% and 16%) respectively. The ZnO NPs preparation was done by sol-gel technique and it was detected by (FTIR), (XRD) and Field (FE-SEM). The anti-bacterial action of ZnO NPs in contrast to P. Aeruginosa was conducted at dissimilar concentrations (100 μg/mL, 50 μg/mL, 25 μg/mL and 12.5 μg/mL). Which were examined in contrast to PDR, XDR and MDR isolates, the effects showed that the highest inhibition area of isolates was at concentration 100 μg/mL and the lowermost inhibition area was at concentration 12.5 μg/mL. The minimum inhibition concentration of the Zno NPs was at a concentrations ranging from 2600μg/mL -162.5μg/mL on PDR, XDR and MDR isolates of the bacterium P. Aeruginosa Keywords: Pseudomonas Aeruginosa, Zinc oxide, Isolation,