The effects of cypermethrin on primary hepatocyte culture of Van fish (Alburnus tarichi Güldenstädt, 1814)

Cypermethrin is a synthetic insecticide widely used in the fight against harmful insects in animal husbandry and agriculture. Although used for combat against insects, it also has negative effects on other harmless animals. Cypermethrin is used in livestock and agriculture fields in Van Lake. Congestion can also cause accumulation in lakes and aquatic areas. In this study, we aimed to determine the effects of cypermethrin on the primary hepatocyte culture of Van fish (Alburnus tarichi). Different concentrations of cypermethrin (1 × 10-7, 1 × 10-6 and 1 × 10-5 M) were applied to the liver cells isolated from Van bean for 48 hours. Changes in Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Lactate dehydrogenase (LDH) levels at 24 and 48 hours of cell culture were determined in the culture medium. We also tried to determine the effects of cypermethrin on total oxidant (TOS) and antioxidant levels (TAS), malondialdehyde levels (MDA) and DNA damage. As a result of the experiments, invert light microscopy revealed that the number of hepatocytes significantly decreased in the groups treated with cypermethrin. Although the levels of AST, ALT and LDH frequently used in liver damage were increased due to the doses of cypermethrin, this increase was low or statistically insignificant compared to the control group. MDA levels increased at the end of the incubation depending on the dose. There was no difference in TAS and TOS levels when compared to the control group. DNA damage was found to increase with time of typhoon and time. As a result, it has been clearly demonstrated that the administered cypermethrin has high toxic effects on the primary hepatocyte culture of Van fish. Keywords: Alburnus tarichi, Cypermethrin, Primary hepatocyte culture, Van Fish