The evaluation of potential markers properties of some antioxidant enzymes and metabolites in patients with polycystic ovary syndrome

Polycystic ovary syndrome (PCOS) is a chronic endocrinological condition that affects about 20% of women of reproductive age and presents a wide spectrum of symptoms such as obesity, menstrual and fertility disorders, hirsutism and insulin resistance for which hyperandrogenism and oligo-anovulation are responsible. Glutathione (GSH), serum gamma-glutamyl transferase (GGT), gamma-glutamylcysteine synthetase (ꝩ-GCS), and aminopeptidases all play a part in eukaryotic oxidant/antioxidant systems. In this study, we aimed to show that glutathione and related enzymes can be an important antioxidant/oxidant indicator in PCOS disease. In our study, among the patients followed and being followed in Gynecology; Forty patients over 18 years of age, diagnosed with PCOS according to 2003 Rotterdam diagnostic criteria, and 40 healthy volunteers without any metabolic disease were included. Rotterdam diagnostic criteria; oligo-anovulation, clinical and/or biochemical hyperandrogenism and ultrasonographically polycystic ovary appearance. People who had at least 2 of the three criteria were considered PCOS. The GSH, GGT, ꝩ-GCS and aminopeptidase levels were measured by the ELISA method in serum samples of patients and controls. In our study, the levels of important enzymes in glutathione metabolism were examined in PCOS patients. These enzymes were found to be significantly different in PCOS patients compared to control groups, indicating that glutathione metabolism plays an important role in this disease. When correlation analyses are analyzed, the correlation of the parameters shows that enzymes and glutathione play a role together in the disease. According to the analysis results; GGT was found to be insignificant between PCOS and control (p>0.05). GSH, ꝩ-GCS and aminopeptidase were found to be statistically significantly higher in the PCOS group compared to the control group (p<0.05). Since GSH is an important antioxidant, it is thought to increase in response to the increase in oxidants. Since ꝩ-GCS and aminopeptidase are involved in the catalysis of glutathione, it is thought to increase in response to glutathione elevation. In the light of the data we have obtained, we think that glutathione will play an important role in the mechanism of PCOS. The PCOS is an important disease that can be seen in many women and seriously affects the quality of life. Therefore, it is important to clarify the reasons behind the spread of the disease clearly and thus a better diagnosis. More studies can be done to better clarify the relationship between glutathione metabolism and PCOS. Further, the relationship between the phenotypes can be clarified by taking different PCOS phenotypes. Also, differences between patients with different outcomes can be examined. Differences can be examined by measuring the gene expression of the enzymes that we examined, and in this way the relationship with the disease can be better clarified.