Fare preantral foliküllerinin üç boyutlu ve iki boyutlu hücre kültürü ortamında büyümesi ve yaşaması

In vitro ovarian culture is a new frontier in assisted reproductive technology with tremendous potential, especially for fertility preservation. Folliculogenesis within the ovary is a complex process requiring interaction between somatic cell components and the oocyte. Conventional two-dimensional culture of ovarian follicles disrupts spherical growth and spatial arrangements between oocyte and surrounding granulosa cells. Granulosa cell atachment and migration can leave the oocyte naked and unable to complete the maturation process. Recognition of the importance of these arrangements between cells has lead to research in to three-dimensional culture system. In the present work preantral follicles isolated form 14 day old immature mouse ovaries were cultured either 3 dimensionally using matrigel or using 2d conventional culture. The result suggests that matrigel culture provides better milieu for growth and maturation of mouse preantral follicles.