Effects of rosmarinic acid in ovo injection in broiler hatching eggs treated with different formaldehyde fumigation on incubation results and post performance

In this study, The aim of this study was to examine the effects of in ovo feeding method and rosmarinic acid injection on hatching results and post-emergence performance results on broiler hatching eggs with formaldehyde disinfection at different rates (F3x and F4x). A total of 720 Ross 308 fertile broiler hatching eggs were used in the experiment. Trial design of the study 4 treatment groups in 2 main groups (RK-: No application, RK+,: 0.5 ml isotonic water, R1: 0.5 ml 250 ppm rosmarinic acid, R2: 0.5 ml 500 ppm rosmarinic acid) and 3 replications in each treatment group. On the 18th day of incubation, the air sacs were determined from the blunt side of the eggs and injected into the amniotic fluid at a depth of 18 mm from the side of the air sac. After the completion of the incubation process, a sufficient number of hatched chicks from each treatment group and a total of 240 chicks were transferred to the experimental house. The chicks were reared for 6 weeks and data on the developmental performance of the animals were collected during this period. As a result, the effect of formaldehyde fumigation dose (F3x, F4x) on late term mortality (%) and hatchability (%) was found to be statistically significant (P<0,05). The effect of in ovo injection of rosmarinic acid on hatching weight (g) and hatchability (%) was found to be statistically significant (P<0,05). The effect of formaldehyde fumigation dose and rosmarinic acid in ovo injection on body weight at the end of the trial was found to be statistically significant (P<0,01). The effect of rosmarinic acid in ovo injection on the end-of-trial feed efficiency was found to be statistically significant (P<0,01). The effect of formaldehyde fumigation dose (F3x, F4x) on liver weight in males and females was found to be statistically significant (P<0,05).