Investigation of antioxidant effect of pollen in scaly carp (Cyprinus Carpio Linnaeus, 1758) in different water temperature

In this study, it was aim to investigate changes in some immunological and antioxidant parameters of pollen treated scaly carp (Cyprinus carpio) in different temperatures. For this purpose, fish were stocked to six different glass aquarium which was adjusted to 23 °C water temperature with adjustable thermostat heaters. After fifteen days adaptation period, temperatures of the two aquarium were dropped to 18 °C, while temperatures of the other two were upgrade to 28 °C. These groups were given to % 2,5 pollen for 15 days. Blood and tissue (liver, kidney, and gills) samples were collected at the end of the experiment and analysed to determine the immunological parameters (oxidative radical production (nitroblue tetrazolium-NBT activity), total plasma protein and total immunoglobulin levels) and oxidant/antioxidant status (malondialdehyde and reduced glutathione levels and glutathione-S-transferase activity). The NBT activity, the total protein and total immunoglobulin levels in the group (23 ºC + Po) that maintained at the same temperature with the control group (23 º C) and applied pollen were increased (p <0.05). When compared to the control group (23 ºC), a statistically significant increase in the NBT activity of the groups at 18 ºC was observed (p < 0.05). Decrease in the NBT activity of the group at 28 º C was statistically insignificant (p > 0.05). The total protein and total immunoglobulin levels of the group at 18 º C were decreased (p < 0,05), while increase in the total protein and total immunoglobulin levels of the group at 28 º C was not significant (p > 0,05). When compared to the control group (23 ºC), the NBT activity, the total protein and total immunoglobulin levels in the groups (18 ºC + Po ve 28 ºC + Po) that maintained at the same temperature with the control group (23 º C) and applied pollen did not show any statistically significant difference (p > 0.05). The tissue MDA levels in the group (23 ºC + Po) that maintained at the same temperature with the control group (23 º C) and applied pollen were decreased (p <0.05). The tissue MDA levels were increased in the groups at 18 °C and 28 °C when compared to the control group (23 º C) (p < 0.05). The tissue MDA levels of the groups (18 ºC + Po ve 28 ºC + Po) treated pollen simultaneously with the change in temperature were lower than the groups at 18 °C and 28 °C. The tissue GSH levels and GST activities in the group (23 ºC + Po) that maintained at the same temperature with the control group (23 º C) and applied pollen were increased (p < 0.05). The tissue GSH levels and GST activities were decreased in the groups at 18 °C and 28 °C when compared to the control group (23 º C) (p < 0.05). The tissue GSH levels and GST activities of the groups (18 ºC + Po ve 28 ºC + Po) treated pollen simultaneously with the change in temperature were higher than the groups at 18 °C and 28 °C.