The effect of fluvoxamine on the xenobiotic metabolizing enzyme activities and antioxidant system in rainbow trout (Oncorhynchus mykiss)

Fluvoxamine is a drug used to treat depression and anxiety disorders. It is present in aquatic systems due to widespread usage. But, the effect of fluvoxamine on the aquatic organism is not well known. In this study, we aimed to determine the effect of fluvoxamine on CYP1A-associated 7-ethoxyresorufin O-deethylase (EROD), CYP3A-associated erythromycin N-demethylase (ERND), glutathione S-transferase (GST) and antioxidant enzymes, catalase (CAT) and glutathione reductase (GR) activities in rainbow trout (Oncorhynchus mykiss). For this purpose, 24 fish were divided into three groups as control group, 10 microgram/L fluvoxamine administration group, and 50 microgram/L fluvoxamine administration group. Fish were treated with indicated doses of fluvoxamine for 96 hours. At the end of the experimental period, fish were killed and livers were taken. Microsomes and cytosols were prepared and enzyme activities were measured in these fractions. The EROD activity results of the 10 microgram/L fluvoxamine administration group and the EROD activity results of the 50 microgram/L fluvoxamine administration group were found to be significantly different from the EROD activity results of the control group. The GST activity results of the 50 microgram/L fluvoxamine administration group were found to be significantly different from the GST activity results of the control group. The GR activity of the 50 microgram/L fluvoxamine administration group was found to be significantly different from the GR activity results of the control group. No difference was found in ERND and CAT activities between the groups. The results of this study showed that fluvoxamine administration affected some of the enzyme activities in rainbow trout.