The analyis of the effects of metranidazole+amoxicillin treatment on the total antioxidant capacity and superoxide dismutase enzyme activity levels in serum and saliva in individuals with generalize chronic periodontitis

The aim of this study is to analyze the effect of metranidazole and amoxicillin (MET+ AMO) therapy in combination with full mouth scaling and root planning (FMSRP) on total antioxidant capacity (TAOC) and the activity levels of superoxide dismutase (SOD) enzymes in the serum and the saliva of the patients with chronic periodontitis. Fifty patients with chronic periodontitis and 22 periodontally healthy individuals were included in the study. Patients with periodontitis were divided into two groups; the Placebo Group which received only FMSRP therapy and the Antibiotic Group which received combined metranidazole + amoxicillin therapy together with FMSRP. Clinical periodontal parameters and the TAOC and SOD concentrations in the serum and the saliva were measured at the beginning, first and third month of the therapy. The periodontal parameters was significantly lower in the control group than the periodontitis groups and at the first and third month of the therapy as compared with the beginning in the peridontitis groups (p<0.05). Although no significant differences were found in probing pocket depth (PPD) and clinical attachment level (CAL) at the first and third month between the periodontitis groups (p>0.05), in the antibiotic group gingival index (GI) and gingival bleeding index (GBI) were significantly lower than the placebo group (p<0.05). In the antibiotic group, serum TAOC levels were significantly higher at the first and third month than at the beginning (p<0.05) while no difference was observed in the placebo group (p>0.05). In the periodontitis groups SOD levels decrease at the third month as compared with the beginning and this decrease was more prominent in the antibiotic group (p>0.05). Systemic MET+AMO application together with FMSRP decreases the inflammation, it might improve the oxidative stress by causing systemic increase in TAOC and decrease in SOD. Key Words: Reactive Oxygen Species, Oxidative Stress, Chronic Periodontitis, Nonsurgical Periodontal Therapy, Combined Antibiotic Therapy