Effects of vitamin E and Selenium use on antioxidant parameters in Saanen goats under heat stress

Objective: The study was conducted to determine the effects of Vitamin E and Selenium use on antioxidant parameters in Saanen goats under heat stress. Material and Methods: In the study, 14 healthy female goats of the Saanen breed, aged 1 year and above, were selected according to the results of clinical and laboratory examinations (complete blood count and serum biochemical findings). These goats were randomly distributed to the groups and divided into two groups as Group 1 (n=7) and Group 2 (n=7). The animal groups were taken to separate paddocks and their care and management were provided with total mixed ration and adlibitum water without changing their feeding regimes during the study. Vitamin E-Selenium (Yeldif, Ceva, Turkey) was applied to the goats in Group 1, at a dose of 2 ml yeldif/50 kg live weight, and 2 ml 0.9% NaCI was applied to the goats in Group 2 as a single dose subcutaneously in the first week of each month. In order to determine total oxidant level (TOS), total antioxidant level (TAS) and thiol disulfide levels, 4 ml blood samples were taken from the jugular vena into lithium heparin tubes from the animals in both groups. All sampling and applications were monitored once a month for 12 months (seasonally). Results: In this study, it was investigated whether the application of Vitamin E and Selenium had a significant effect on the changes between environmental temperature-humidity index (SNI) and antioxidant parameters (TTL, NTL, DS, TAS, TOS) in Saanen breed goats followed for 12 months. In this study, it was determined that heat stress occurred in the summer season when SNI values increased. TTL levels, which fluctuated over time, were found to be higher in the study group, especially in March and October, compared to the control group. NTL levels, which showed a significant increase especially in the control group in February, did not show a significant change in the other months. The antioxidant effect of Vitamin E and Selenium application was revealed by the significant decrease in DS level at the end of the year. Although TAS and TOS levels showed fluctuations in both groups during the year, no significant change was formed and they showed a similar course. In the regression analysis performed between SNI and antioxidant parameters, a positive and significant relationship (p = 0.048) was detected only between TTL and SNI. Conclusion: In this study, although there were changes between the study and control groups in all oxidative stress parameters, it was determined that these changes were not significant. Keywords: Vitamin E, Selenium, heat stress, TAS, TOS, thiol disulfide, goat