Investigation of optical properties of erythrocyte and other bio-organic particles by using mie scattering

ABSTRACT MSc THESIS INVESTIGATION OF OPTICAL PROPERTIES OF ERYTHROCYTE AND OTHER BIO-ORGANIC PARTICLES BY USING MTE SCATTERING Mehriban YUSIFOVA DEPARTMENT OF PHYSICS INSTITUTE OF NATURAL AND APPLIED SCIENCES UNIVERSITY OF ÇUKUROVA Supervisor: Prof. Dr. Emrullah Mehmetov Year: 2001, Pages: 79 Jury: Prof. Dr. Emrullah Mehmetov Prof. Dr. Süleyman Güngör Prof. Dr. Viktor Pogrebnyak The light scattering and absorption properties of media (or particles) and their analysis are the important problems in many areas of biomedical optics, in particular for determining cell and subcell structures, like erythrocyte, hemoglobin, oxyhemoglobin and etc. It is well known that the investigation of properties (intensity, polarization, dispersion and etc.) of the scattered waves give us more detail information about morphological and physiological features of cells and their changes under influence of the internal and external factors. Various theoretical methods and corresponding computer programs exist which can be used to compute electromagnetic scattering by a cluster of several arbitrarily shaped (ellipsoid, sphere, Chebyshev particle, paraboloid, and etc) particles. Our investigation dedicated to problem of light scattering by a discoid (red blood cell) on a substrate, which is treated using Mie's solution for scattering by a spheroid in a homogeneous medium and a T-matrix method for calculating the scattering characteristics of spheroidal particles, like red blood cell. In this work the theoretical scattering solution for a plane wave incident normal an a lossy dielectric discoid (a/b=2) is obtained. Also scattering phase matrices are calculated for some orientations of discoid. Computational results and model show that there is general agreement for the internal and external intensity, far and near field functions for discoid with the same overall dimensions. Key Words: Light Scattering, Mie Scattering Theory, T-matrix Method, Chebyshev Particles, Erythrocytes. n