The Comparing of sphingosine 1-phosphate 4 gene expression in patients with neuroblastoma to healthy children

Aim: To compare the expression level of Sphingosine 1-phosphate 4 gene caused to cell migration and inhibition of apoptozis in patients with neuroblastoma to healthy children.Material and Method: 37 patients diagnosed with neuroblastoma and control group included 25 healthy children were studied in Cukurova University Pediatric Oncology Department in between January 2007 and January 2011. After seperationing of white blood cells from blood samples, RNA isolation and cDNA were performed.Results: 21 (56,8 %) girls and 16 (43,2 %) boys were diagnosed with neuroblastoma. 25 (67,6 %) patients were under 4 years old and 12 (32,4 %) were 4 years old and above. 81,1 % (30) of tumor was localized on surrenal. 4 (10,8 %) patients were stage I, 2 (5,4 %) stage IIb, 2(5,4 %) Evre III, 28 (75,7 %) stage IV and 1 (2,7 %) patient was stage IVS. After 4 course chemotherapy at first evaluation, 9 (24,3 %) patients CR, 1 (2,7 %) VGPR, 13 (35,1 %) PR, 9 (24,3 %) NR and 1 (2,7 %) PD were found. 11 (29,7 %) patiens were on consolidation phase, 5 (13,5 %) were on induction therapy and 16 (43,2 %) were following up without chemotherapy. 4 (10,8 %) patients were not began chemotherapy. After second evaluation, 1 (9,1 %) patient CR, 9 (81,8 %), 1 (9,1 %) PD were seen in 11 patients with consolidation phase. 20 (54,1 %) of patients were living with disease and the remaining was living without disease. One patient was died. While the mean expression level of Sphingozin 1-phosphate 4 gene was 0,0387±0,0647 in neuroblastoma patients, 0,0366±0,0238 was found in healthy children. The relationship between neuroblastoma patients and healthy children was different as statistically (p=0,028).Patients on consolidation phase had 0,0230±0,0148 for sphingosine 1-phosphate 4 gene expression level. This level was low for that of patients followed up without treatment The difference between two group was significant statistically (p=0,023).Conclusion: The high level of Sphingosine 1-phosphate 4 gene expression in neuroblastoma patients according to healthy children could be referred to over expression of sphingozin 1-phosphate 4 gene in sick children. Therefore, the migration of cancer cells and/or the inhibition of apoptozis could be come up with this result. In addition, the suppression of sphingozin 1-phosphate 4 gene expression level during consolidation phase and the increasing of expression level in following up without chemotherapy meant that the chemotherapy caused the decreasing of cell migration and/or induction of apoptozis.Key words: Neuroblastoma, sphingosine 1-phosphate 4, cancer, migration, apoptozis