Genome-wide approaches for abiotic stress tolerance in P. vulgaris: Gata gene-specific bioinformatic analyzes and evolutionary processes

Transcription factors (TF) are proteins that can bind to DNA by recognizing specific sequences within the promoter regions on the target gene. TF's have an active role in plant development, circadian rhythm, protein interactions and resistance of different plant species against various diseases. The GATA transcription factor is a group of proteins found in almost all tissues of the plant, regulating organ formation and morphogenesis, germination, synthesis of chlorophyll pigment, and responses to environmental stress factors. The GATA transcription factor has a highly conserved type IV zinc finger structure. In this thesis study, 32 GATA gene family members were identified in the bean plant. Exon-intron structure of determined PvGATA genes, distribution on chromosomes, conserved motif structure, phylogenetic relationship, tandem and segmental gene duplications, syntenic relationship between 2 selected different species and bean plant, cis-element analysis, three-dimensional protein homology analysis and miRNA analyzes were performed. Expression levels of genes containing stress-related cis-elements and selected according to RNA-seq data analysis were examined by qPCR analysis. According to the data obtained as a result of all analyzes, the structure, function, conserved regions of PvGATA genes, the responses developed under abiotic stress were tried to be explained, and information was obtained about the evolutionary process of GATA genes in bean with the results of gene duplications and phylogenetic analysis. It is thought that the data obtained in the thesis study can help future studies.