Effects of beta-D glucan on peritoneal immune defence signs and cytokine response in an experimental rat pneumoperitoneum model

Name: Effects of beta-D glucan on peritoneal immune defence signs and cytokineresponse in an experimental rat pneumoperitoneum modelWriter: Dr.Okan YumuşSUMMARYOur knowledge about effects of laparoscopic surgery on peritoneal and systemicimmune response is still lacking. In order to restore the immundisregulationdeveloped by surgical trauma, sveveral methods are being tried. Aim of this study isto investigate the effects of beta-glucan on peritoneal immune system and cytokineresponse in rat pneumoperitoneum model.Twenty eight rats were divided into three groups like; group 1, n=9 (sham- notoperated); group 2, n=9 (control, 10 mmHg pneumoperitoneum for 30 min); group 3,n=10 (beta-glucan, 100 mg/kg/day beta-glucan by gavage, for 7 days beforepneumoperitoneum). 24 h after pneumoperitoneum, samples of peritoneal fluid wascollected from all rats and CD4, CD8, CD25 T cells counted bt flowcytometry andlevels of IL-4,IL-10, IL-12, IFN-gamma, TGF-beta measured by ELISA.CD4+CD25+ T cells (Treg) were significantly higher in beta-glucan group fromothers and IL-10 levels were significantly higher in beta-glucan group whencompared to sham (p<0.05). IFN-gamma was higher in control group whencompared to sham and it significantly decreased in beta-glucan group compared withcontrol (p<0.05). There was no difference in CD4+ T cell ratio between all groups butCD8+ T cells were slightly higher in beta-glucan group. TGF-beta was higher in beta-glucan group than others. There was no significant difference in IL-4 and IL-12among all groups.Pneumoperitoneum triggers a peritoneal immundisregulation but beta-glucantreatment reduces the degree of this disregulation by increasing regulatory T cellsand suppress the developing inflammation. This is the first study in the literaturewhich investigates the effects of beta-glucan in experimental laparoscopic surgeryand regulatory T cells. For this reason, to investigate the effects of beta-glucan onthe other components of immune system and to determine the effects developing bydifferent dosage and usage period of this drug further investigations are needed.