Investigation of the effect of quesetin in experimental ischemia-reperfusion injury caused in rat testicle

Aim: Testicular torsion (TT) is the most important cause of childhood acute scrotum. It is an urgent surgical problem that can lead to organ if left untreated. The aim of treatment is to reduce or eliminate torsion/detorsion damage. On of the metods to be used for this purpose is the effect of antioxidants. In this study, it was aimed to prevent experimentally induced torsion/detorsion damage in rats with a unique bioflavonoid and quercetin, an antioxidant known to be aagainst oxidative damage. Material and Methots: In the study, 32 male Sprague Dawley rats, 12 weeks old and weighing 250-300 gr, were used. They were divided into 4 separete groups. No surgical procedure was performed in the control group. 5-hour ischemia was achived by applying torsion to the left testicles in the torsion group. After 5 hours of ischemia, a 2-hour reperfusion was applied to the torsion/detorsion group. In the torsion/detorsion+quercetin group, 2 hours of reperfusion was applied after 5 hours of ischemia and i.p. quercetin was injected. Result: In our study, it was determined that MPO (myeloperoksidaz) and MDA (molondialdehit) values increased significantly in the 2nd group, while the increase was found in the 4th group, which was applied quercetin, but it was not significant. İt has been shown in the 4th group that the mean CAT (katalaz) was low in torsion group and the application of quercetin was effective in the returning the CAT levels to normal. A significant decrease was observed in the quercetin-administrered group in terms of spermatocyte degeneration and inflamation. There was a decrease in dilatation data in the quercetin group, but it was not statistically significant. Conclusion: Quercetin, which has a strong antioxidant effect, has been shown to have positive effects on experimentally induced torsion/detorsion damage. Keywords: Testicular torsion, ischemia-reperfusion, antioxidan, quercetin, Inflammation