Comparison of effects for platelet rich plasma, bone marrow stromal cell and electromagnetic field stimulation superficial temporal fascia used at experimental bone defect model

Reconstruction of bone defects that occur because of any reason has an important place in plastic and reconstructive surgery. The objective of the treatments of these defects is to reinstate the continuity of tissues placed in the area in which the defect has occurred. Spontaneous healing is limited in such large defects and often requires surgical intervention. Autogenous bone graft is one of which are used most frequently in the surgical intervention process. However, studies on alternative methods are carried out since this implementation has donor site complications and has a chance to obtain them in limited sizes.In this experimental study, the effect of the PRP, BMSC and magnetic field implementations which propound that they have a positive impact on bone regeneration were evaluated with the bone recovery speed in the zygomatic bone defect model enwrapped with superficial temporal fascia.After creating a 4 mm defect on the zygomatic bone of the experiments, the defect was encompassed with a superficial temporal fascial flap and a non- union was created. After surgery, different combinations of the PRP, BMSC and electromagnetic field implementations were implemented on the defected area. After implementation, bone regenerations of the experiments were evaluated with hydroxproline, ALP activity and osteocalcin measurements. All the experiments were subjected to bone density measurement. At the end of the experiment the conducted biopsies were histopathologically examined and the bone regeneration rates were microscopically evaluated.As a result of the findings, it was detected that the PRP and PEMF implementation was rather a beneficial and an effective combination in terms of bone regeneration. It was observed that the superficial temporal fascial flap used in the experiment was also a well scaffold choice providing an ideal bone regeneration area because of its autogeneous, vascular and three dimensional structures. As a result, it is thought that this combination in the non-healing bone defects is a rather useful treatment choice and it can be used in a reliable way in clinical applications.