Molecular determination of the existence of grapevine pinot gris virus, grapevine a virus and strawberry latent ringspot virus in vineyard areas of Tokat province

In this study, which was carried out in Tokat in 2021, samples were collected from young leaves and one-year-old shoots of vines showing virus symptoms from some vineyard areas in Merkez, Erbaa, Niksar, Pazar and Zile districts where viticulture is intense. Collected 189 grapevine samples were subjected to RT-PCR test, which is a molecular method using virus-specific primers, in order to detect the presence of Grapevine pinot gris virüs (GPGV), Grapevine A virus (GVA), Strawberry latent ringspot virus (SLRSV). More than one virus was found in 2 (1.05%) of 189 tested samples. Out of a total of 189 plant samples, 80 (42.32%) of GVA, 3 (1.58%) of GPGV were detected and SLRSV (0%) was not detected. It was determined that the most common virus in the plant samples collected from Tokat Center and its districts was GVA. The least detected viruses were GPGV and SLRSV. Bidirectional analyzes of GVA sequenced isolates were performed and similar phylogenetic tree samples with MEGA10 different aligned and gene local isolates. According to phylogenetic analysis studies, GVA showed different branching within and with different isolates registered in the gene bank. GVA-infected isolates showed similarity with other registered isolates at rates of 92-94%.