Investigation of the effect of turkish propolis on cell cycle

Propolis is a resinous substance produced by honey bees using the clothes they collect from their plants. Since ancient times, propolis has been used as a supplement in the treatment of many diseases and other areas. Antibacterial, antiseptic, antitumoral, anti-inflammatory, antioxidant and anesthetic effects are some of its biological activities that are known and researched. The cell cycle is a cycle that includes phases in which cell growth and division are controlled. Control of the cell cycle is important for tissue homeostasis and cancer. In this study, it was aimed to investigate the relationship between propolis activity and cell cycle in HeLa and HEK293T cells. In our study, cell cycle analyzes were performed by 24 hours in vitro treating HEK293T and HeLa cells with LD50 concentrations of two different commercial forms of propolis produced in Turkey. As a result of cell cycle analysis with flow cytometry; It was determined that propolis 1 statistically significantly reduced the number of cells in the G1 phase by 24% (p=0.004) in HeLa cells and 27.4% in HEK293T cells (p=0.024) compared to the control. It was determined that propolis-2 did not affect the percentage of cells at any stage of the cell cycle in a statistically significant level in HEK293T and HeLa cells compared to the control. Experimental data obtained from our study show that Propolis-1 causes HEK293T and HeLa cells to accumulate in the S-G2/M phases of the cell cycle and arrets the cell cycle.