Molecular characterization of pepper (Capsicum annum l.) genotypes using SSR markers

In this thesis, it is aimed to reveal the differences between pepper varieties using SSR technique which is one of the molecular markers. Pepper is known as a vegetable grown in tropical and subtropical climatic zones of the Solanaceae family. The pepper seedling varieties used in the study were obtained from various seedling companies of Antalya province in the Mediterranean Region. Molecular analysis studies were conducted in 10 varieties of pepper seedlings. For this purpose, samples of genotypes in pepper were taken under appropriate conditions and analyzed in Isparta University of Applied Sciences, Agricultural Sciences and Technologies Faculty, Agricultural Biotechnology Laboratory. As a result of analysis with SSR markers, pepper varieties were divided into 2 main groups according to UPGMA method. The first main group is divided into two sub-groups. In the first sub-group Vezir, Üçburun, Acıburun, Yükselince, Anadol, Serenad, Hayfa Şili is located. In the second subgroup, Jalomex is located. The second main group is Ergenekon and Kanyon. Vezir-Üçburun and Yükselince- Anadol varieties are similar groups and grouped together with the same characteristics in terms of the regions examined. Ergenekon-Kanyon, Serenad-Hayfa Şili is another branch showing similarity among themselves. The most distant similarity is between Jalomex and Ergenekon, the latter being between the similarity Jalomex-Kanyon. It was determined that the total number of alleles was 162, the number of specific alleles was 60, and the band size ranged from 164 to 294 bp. Polymorphic information content (PIC) ranged from 0.04 to 0.89. SSR findings of pepper species in Turkey, creating a step in the selection of the next breeding parents to work, to determine the span of pepper genotypes, the comparison of genetic collection, used in the characterization of pepper genotypes.