Investigation of the effects of vitamin d on renal tissues and TRPV1 channels of doxorubicin administered rats

Doxorubicin is an antineoplastic agent in anthracycline group, and may show nephrotoxic effects in animal models and in humans. in this study we investigated the changes in the kidney tissue with doxorubicin treatment, and the effects of vitamin D on TRPV1 channels. In this study, 24 adult male Wistar Albino rats were used. Animals were divided into 4 groups as 6 animals in each group. During the 14- day experiment period Group I did not have any application. Group II had 10 mg / kg single dose of doxorubicin intraperitoneally (ip); Group III had 10 mg / kg single dose of ip doxorubicin and oral 200 IU / day cholecalciferol; and to Group IV oral 200 IU / cholecalciferol was administered. At the end of the experiment the rats were decapitated and kidney tissues were removed. Tissues were controlled with light microscope and embedded in paraffin blocks. The findings examined and photographed. As result of immunohistochemical staining and examination with light microscope; TRPV 1 immunoreactivity of DXR group decreased in comparison with control group, and vitamin D application did not reverse this effect. Apoptosis detected by the TUNEL method, tended to increase in the doxorubicin group and partly reversed with vitamin D administration. Tissue malondialdehyde (MDA) levels were observed to correlate with the findings of apoptosis. This study showed that vitamin D has anti-apoptotic and antioxidant effects on kidney tissue after doxorubicin induced injury. Keywords: Doxorubicin, kidney, trpv 1, vitamin D.