Evaluation of mirna expression and its relationship with biomarkers in newborn with hypoxic iscemicensephalopathy

In this study, due to the absence of a biochemical test in clinical use that can show the presence, severity, time of onset, and prognosis of HIE, and due to studies in the current literature showing that miRNAs play a central role in brain development and pathogenesis of HIE, the miRNA expression profile in newborns with HIE has been investigated. and we aimed to evaluate the potential of using these miRNAs as biomarkers. They systematically reviewed studies on biomarkers for use as early markers of neuronal damage for HIE. In this thesis, it is aimed to evaluate miRNAs such as miR-210, miR-374'a-5p and miR-21 as potential biomarkers in neonates with HIE. A total of 46 newborns, 23 of whom were diagnosed with hypoxic ischemic encephalopathy, and 23 of whom were healthy controls, were included in the study. Among the newborns whose neonatal encephalopathy we investigated, mir210, mir 374-a, mir 21, TNF-alpha, IL-10, IL-6, IL-1-alpha, HIF-1-alpha levels were diagnosed in the blood samples of newborns 0, 24, and 48. -Worked in the 72nd hour. When evaluated together with the samples and the patient's clinic, the patients included in the study were classified as control, stage 1, stage 2 and stage 3. At the same time, the demographic characteristics of the patients were also discussed. In our study, different biomarker values of hypoxic ischemic encephalopathy (HIE) patients were investigated according to ischemia duration. Accordingly, IL-10 (0. hour, 24. hour and 24-48. hour), TNF-alpha (0. hour, 24. hour and 48-72. hour), HIF-1 (0. hour). , 24th hour and 48-72nd hour), IL-6 (0th hour, 24th hour and 48-72nd hour) and IL-1 (0th hour, 24th hour and 48-72th hour) ) biomarker values have increased In our study, miRNA expression and biomarkers of the patient group were compared with the control group. Among the miRNA and biomarkers examined, mir210, mir374-a-5p, mir21-5p, IL-10, HIF-1-a, IL-6 and IL-1-a levels were found to be significantly higher in the patient group. However, TNF-alpha levels increased in the patient groups compared to the control group, but there was no statistically significant difference between the two groups (p=0.46). In our study, we evaluated the relationship of certain miRNA expression values and inflammatory biomarkers with disease stages in patients with hypoxic ischemic encephalopathy (HIE). Between disease stages in terms of both miRNA expressions (mir210, mir374-a-5p, mir21-5p) and certain inflammatory biomarkers (IL-10, TNF-alpha, HIF-1a, IL-6, IL-1a) significant differences were found. In particular, the expression levels of mir210 (p<0.04) and mir374-a-(p<0.001) were found to be significantly higher in stage 1, 2 and 3 groups compared to the control group. However, although the expression level of mir21-5p was increased compared to the control group, it did not show a statistically significant difference (p=0.06). In our study, miRNA expression values of hypoxic ischemic encephalopathy (HIE) patients were compared according to ischemia duration. According to our results, mir210 (0. hour, 24. hour and 48-72. hour), mir374-a-5p (0. hour, 24. hour and 48-72. hour) and mir21-5p (0. hour) , 24th hour and 24-72nd hour) miRNA expression increased. As a result, in this study, it was observed that the expression of serum miRNAs was increased in a hypoxia-sensitive manner. These serum miRNAs were evaluated as potential biomarkers in the future.