Investigation of the effects of Nerolidol and vitamin D in rats with isoproterenol created with experimental myocardial infarction

Cardiovascular diseases are one of the leading causes of death globally. The mortality and morbidity rates of patients with acute myocardial infarction (MI) are 1.5 times higher than in the general population. Nerolidol (NRD) is a naturally occurring sesquiterpene alcohol found in the essential oil of various floral-scented plants. Vitamin D (VIT D) has many important functions in metabolism, especially in the bone and skeletal system. Studies have shown that NRD and VIT D can be used to treat cardiovascular diseases and hypertension. 42 numbers of 8-10 week old Wistar Albino male rats were included in our study, and they were divided into 6 groups with 7 rats in each group. Working groups include Control, MI, NRD, NRD+MI, VIT D, MI+VIT D. 200 mg/kg Isoproterenol was used to induce acute MI. Rats were treated with NRD dissolved in corn oil (100 mg/kg/day) and VIT D (50 IU/day) for 14 days. The therapeutic effects of NRD and VIT D were examined with cardiac markers, oxidation and biochemical parameters, and histopathological and immunohistochemical methods. As a result, MI+NRD and MI+VIT D groups, compared to the MI group, increased the total antioxidant status(TAS) and decreased the total oxidant status(TOS) level significantly (p<0,05). Asprosin levels were significantly lower in the MI+NRD and MI+VIT D groups compared to the MI group (p<0,05). Meteorin Like Protein (METRNL) levels significantly increased in the MI+NRD and MI+VIT D groups compared to the MI group (p< 0,05). In histopathological examinations, it was observed that erythrocyte extravasation, and fibrosis were significantly reduced, and necrotic muscle fibers showed a significantly improved pattern in the MI+NRD and MI+VIT D groups compared to the MI group. (p<0,001, p=0,002, respectively). Nerolidol and Vitamin D were found to be effective in the treatment of MI, and this effect was dependent on METRNL but independent of asprosin.