The seasonal variations in fatty acid compositions in different tissues (muscle, liver, gonad) of red-spotted trouth salmo trutta macrostigma (Dumeril, 1858) (Osteichthyes: Salmonidae)? collected in Munzur river

In this study, the sexual and seasonal variations in total lipid and fatty acid compositionof total lipid, phospholipid and triacylglycerol fractions in muscle, liver and gonads of male andfemale red - spotted trout Salmo trutta macrostigma was investigated. Fish samples have beencaught from the Munzur River in two-month periods between november 2009 and september2010.The total lipid content varied seasonally from 1.44 % to 1.90 % in female muscle, 1.07% to 2.45 % in male muscle, 2.62 % to 5.60 % in female liver, 3 % to 4.64 % in male liver, 2.90% to 5.31 % in female gonad, 2.88 % to 4.12 % in male gonad of wet weight for S. t.macrostigma. It was determined that liver tissue contained higher percentages of total lipid thanmuscle and gonad tissues of S. t. macrostigma The total lipid amount in tissues of S. t.macrostigma was influenced by reproduction period, temperature and season.Fatty acid compositions of total lipid, phospholipid and triacylglycerol fractions havebeen determined in muscle, liver and gonad tissues of fishes by gas chromatography using amixture of fatty acid standards.The present study revealed that ?SFA, ?MUFA, and ?PUFA levels varied among bothsexes and different tissues of the fish. The ?SFA and ?MUFA percentages of the total lipidextracted from tissues of the both sexes ranged from 26.23 % to 44.13 % and from 17.42 % to37.48 %, respectively. The ?PUFA percentages of the total lipid in tissues was found between23.98 % to52.34 % .Generally, ?PUFA percentages of the total lipid in fish tissues were foundhigher than ?SFA and ?MUFA of both sexesThe distributions of ?SFA, ?MUFA and ?PUFA proportions were found differentamong total lipid, phospholipid and triacylglycerol fractions from lipids in tissues of fish in allseasons. Triaçilgliserol contained a lower proportion of ?PUFA and a higher proportion of?MUFA and ?SFA than PL while PL contained higher proportion of ?PUFA than proportion of?MUFA and ?SFA compared to TAG.The main constituents were C16:0 (17.25 - 31.86 %) among SFA, C18:1n-9 (13.87 -26.80 %) among MUFA, 22:6n-3 (6.43 - 24.35 %) and 20:5n-3 (5.06 ? 13.08%) among PUFAin the total lipid. Palmitic acid (16.49 - 40.66 %) among SFA, and C18:1n-9 (18.76 - 36.42 %)among MUFA, 18:2n-6 (3.40 ? 29.10%), 18:3n-3(1.36 ? 11.80%), 22:6n-3 (1.53 - 22.38 %) and20:5n-3 (1 ? 10.19%) among PUFA in TAG. Palmitic acid (20.76 - 41.90 %) among SFA,C18:1n-9 (8.44 - 27.28 %) among MUFA, 22:6n-3 (7.29 - 28.78 %) and 20:5n-3 (4.82 ? 15.01)among PUFA in PL extracted from tissues of S. t. macrostigma in all seasons.Triacylglycerols were characterized by a high content of ?MUFA, ?SFA, fatty acidsuch as 14:0, 16:1n-7, 18:2n-6 and 18:3n-3; whereas phospholipids contained a large quantity of?PUFA, mainly, 20:4n-6, 20:5n-3, 22:5n-3, 22:6n-3 and 16:0 and 18:0 among SFAs.Generally, 16:0 percentages of the PL in fish tissues were found higher than TAG ofboth sexes.The 16:0 percentages of PL and TAG fractions in muscle, liver and gonad tissues ofthe both sexes ranged from 20.76% to 41.90 % and 16.49% to 40.66 %.It was found that the percentage of 18:2n-6 in the total lipid and both fractionsextracted from muscle, liver and gonad of both sexes was higher in september than to othermonths.In this study, the n-3/n-6 ratio was determined to range from 2.79 to 5.79, for dorsalmuscle, 2.73 to 4.82 for liver and 1.86 to 4.33 for gonads in total lipids in both sexes of studiedfish.The results indicated that S. t. macrostigma were excellent sources of polyunsaturatedfatty acids with very high levels of ?-3 fatty acids, especially eicosapentaenoic anddocosahexaenoic acids and it may be a valuable food source for human consumption in terms offatty acids.