Examination of serume GSTP1 isoenzyme changes at pediatric cardiac operations and evaluation of relation of this changes to early postoperative morbidity and mortality in

In modern era, most of the cardiac operations are performed under cardiopulmonary bypass(CPB). In the course of cardiopulmonary bypass, contact of the blood to foregin surface, ischemia end reperfusion damage, heat change and duration, endotoksins and operational trauma provakes release of pro-ınflamatory agents and this agents cause systemic ınflammatory response syndrome (SIRS) that causes pulmonary, renal, haemostatic and cerebral failure. Despite the developments of anesthesia, surgical and perfusion techniques SIRS is still a majör cause of morbitity and mortality after cardipulmonary bypass. Thereof, a new marker for defininition of prognozis and cardiopulmonary bypass releates mortality is needed. Oxidative stress is one of the major cause of ınflammatory response. Glutathione S-Transferase P1 (GSTP1) isoenzyme, one of the main protection systems against oxidative stress, may be defective in pathological conditions such as CPB and this may cause negative results fort he organism. Besides GSTP1 isoenzym is to shown to be related with cardiac failure. With the enlightment of this facts, we aimed to examine the changes of GSTP1 in early postoperative period, define the poor prognozis for starting the agressive therapy to prevent mortality and morbidity. In this prospective study 30 pediatric patients that underwent to CPB were examined. Patints were divided in to two groups as discharged with no problems (Group 1, N=23) and exitus(Group 2,N=7) groups. During induction of anesthesia, after 10 minutes from staring of CPB, after 10 minutes from starting of cross-clemping, 30 minutes later from end of CPB and postoperative 8. hours venous blood samples were collected. Samples were examined with Human GSTPi ELİSA Kit. Then results were corelated to creatinine levels The finding showed that; creatinine levels were significantly elevated at phase 5, but GSTP1 levels were elevated at phase 4. This results shows that; GSTP1 evaluation may be an early prognosis indicator after CPB.