Experimental investigation of the effects of probiotic supplementation on intestinal permeability and diabetes-associated parameters in mice with disrupted circadian rhythms

In our study, in animal models whose circadian rhythms were disrupted by changing the light/dark cycles experimentally, probiotic supplementation; It was aimed to investigate the effect on diabetes and intestinal barrier permeability markers. Three different experimental animal groups, each consisting of 8 Balb/c mice, were formed. The first group was the control group, the second study group was the group whose circadian rhythms were disrupted by changing the light/dark cycles, and the third study group was the group whose circadian rhythms were disrupted by changing the light/dark cycles and were supplemented with probiotics from week 8 to week 16 (end of the study). All three experimental animal groups were fed with standard chow throughout the study, and weight changes were followed weekly. ELISA method was used for biochemical analysis. As a result of our study, statistically significant differences were observed between the groups with impaired circadian rhythms in weight changes between the initial and final weights (p<0.05). In mice with impaired circadian rhythms, insulin and HOMA-IR values were increased compared to the control group, and QUICKI value decreased (p<0.05). Changes in fasting blood glucose and zonulin levels were not statistically significant (p>0.05). It was concluded that probiotic supplementation had a positive effect on weight gain, serum zonulin levels, Insulin, HOMA-IR and QUICKI levels in mice with impaired circadian rhythms.