Molecular Characterization of Dicotoculus filaria in Sheep of Van Province

This study aimed to determine the molecular characterization of Dictyocaulus filaria, known as lungworm, obtained as a result of necropsy examination from sheep brought to the municipal slaughterhouse of Van province for slaughter.In the genomic DNA of Dictyocaulus filaria species used as material in the study, 28S rRNA gene region was amplified by PCR.In the agarose gel, 952 bp length amplicons specific to the 28S rRNA gene region were detected. Next, the amplicon sequence obtained from bidirectional sequence analysis from a PCR product was compared with reference sequences in Genbank by BLAST.By comparing the genotype obtained from the isolate with its exact or closest similarities, phylogenetic tree was created using the Neighbor-Joining method with the MEGA 7 program.As a result of the sequence analysis, it was revealed that Dictyocaulus filaria obtained from sheep was 100% similar.As a result, DNA sequencing analysis of 28S rRNA gene region of D. filaria, known as lungworm type, which is frequently encountered in Van region sheep, has been revealed with this research.