Determination of Meca gene presence in MRSA strains isolated from intensi̇ve care by conventional, automated and PCR method

Meticillin-resistant S. aureus (MRSA) strains are becoming increasingly important as a cause of hospital and communityacquired infections. The aim of this study was to compare the presence of mecA gene, a genotypic method, by different methods used to determine methicillin resistance in S. aureus strains. The study included 50 MRSA strains isolated from various samples (wound, blood, sputum, respiration, sputum, abscess, osteomyelitis, etc.) from the microbiology laboratory of Yüzüncü Yıl University Medical Faculty between 2010-2016. A single isolate was obtained from each patient. In our study, the presence of mecA gene in MRSA strains was investigated by conventional, automated and PCR method. In the detection of mecA gene in the MRSA strains examined, the presence of mecA gene was found in 49 of 50 MRSA strains examined by Oxacillin E-test method (MIC> 2); The presence of mecA gene was detected in all of the samples examined by cefoxide E- test method (MIC> 4). All of the samples examined by Vitek 2 Automated method were found to have mecA gene. All samples examined by PCR method were positive, the presence of mecA gene was determined. Sensitivity rate of strains examined by oxacillin E-test method was 98%, while the susceptibility rate of strains examined by Cefoxide, Vitek 2 Automated and PCR methods was 100%. In order to obtain the necessary precautions in hospitals with high MRSA ratio, mecA investigation is of great importance in achieving the correct results. Since the results of the