Investigation of the effects of high intensity therapy ultrasound (HİTU) on invitro glial tumor cell cultural environment

Purpose: Brain tumors are common pathologies in the society, in which the life expectancy of the patient cannot be extended despite the application of surgical intervention, radiotherapy and chemotherapy. Radiotherapy-like treatment methods, which have high risks and affect the quality of life of the patient, may cause serious complications and even life risks. These negative effects both reduce the life comfort of the patient and cause serious distress in his social environment. In addition, the treatment of complications causes a serious cost and makes it difficult for institutions in countries with limited budgetary resources to cope with this financial burden. Developing alternatives to treatment methods with high post-application complications such as radiotherapy will partially reduce the mentioned problems. In this study, we examined the effect of High Intensity Area Ultrasound (HIFU), which is a new non-invasive treatment method and does not cause early and late complications such as radiotherapy, on the structural integrity of these cells and the possible molecular pathways involved in this after laboratory application. Equipment and Methods: After the glioblastoma cells were grown by culture, they were divided into 6 groups in equal amounts. No treatment was given to the first, second and third groups. These three groups were subjected to microscopic examination, cell count by flow cytometry and ATP activity and cell viability by ATP proliferation kit, respectively, at three different stages, immediately after the incubator, after 4-6 hours and finally after 24 hours. In groups four, five, and six, after HIFU application, it was followed by microscopic examination, cell count with flow cytometry and ATP proliferation kit in three different stages: immediately after the incubator, after 4-6 hours and finally after 24 hours. vitality was subjected to examinations. HIFU and no treatment groups were compared. Results: While no significant change was observed in the cell groups treated with HITU immediately after 4-6 hours and 24 hours after the phase contrast microscopy examination, it was observed that the tumor cells did not adhere to the surface and there was a significant decrease in the number of cells. When the treated and untreated groups were compared, it was observed that the number of viable cells decreased over time by counting cells with flow cytometry in the treated group. It was observed that the ATP fluorescence density decreased significantly with the number of cells over time in the groups where ATP activity and cell viability were analyzed with the ATP proliferation kit. Conclusion: As a result of microscopic examination, flow cytometry cell count and ATP activity studies in all 3 groups where we applied HITU, it was observed that the cells did not maintain their vitality. In the control groups, continued cell viability was observed by microscopic examination, flow cytometry and ATP activity. In this study, the lethal effect of HITU on Glioblastoma cells as a noninvasive treatment method has been demonstrated at microscopic and molecular level.