Alternatif akım kutupsal devinim ile sürekli akışta polistren parçacıkların yönlendirilmesinin comsol simulasyonu

In the near feature, we will see complicated and sophisticated Lab-On-a-Chip (LOC) devices for many kinds of medical application, which will fit on ones palm and does the job of a high tech laboratory. For enhancing such kinds of devices, the physical and chemical truths, theories, phenomenon will interlace with micro & nano technology. Dielectrophoresis (DEP) is a non-destructive, electro kinetic transport mechanism with potential for the separation and manipulation of bio particles in microfluidics devices. With the developments in the Microelectromechanical systems (MEMS) and nanotechnology, a large electric field with weak voltages can be obtained for manipulating, separating, and characterizing micro/ nano-sized particles such as cells, Deoxyribonucleic acid (DNA), proteins, nanotubes, and nanoparticles. In order to make adequate devices the design and simulation should well correlated to get most feasible and optimum geometry of the electrodes and the micro channel network. Basics of the DEP simulations lies on proper estimation, characterization and usage of Electro hydrodynamic forces and their effects. Simulation generally involves the electrical potential field, the flow field and the particle tracing. A three Dimensional (3D) model of the DEP chip on Multiphysics software can do simulation of these physical results. In this thesis work, angular interdigitated electrodes and Polydimethylsiloxane (PDMS) channels designed to separate the polystyrene particles and two different Comsol simulations has been performed. In the first simulation, a basic model that has only two electrode couples is used. A statistical approach known as surface response is used to obtain an optimization model for this simple model with the results obtained from Comsol simulation. An equation is generated and optimized running conditions identified for the simple model. In the second simulation, a realistic model, which has 43 electrode couples, is used. Same boundary conditions, initial conditions and estimations applied in both model. Electrostatic, flow and particle tracing modules used in Comsol simulations and results obtained for a parameter group. After that, fabrication of the LOC device explained in details and experimental setup introduced. Before the experiments, Bovine Serum Albumin (BSA) solution with Phosphate Buffered Saline (PBS) is pumped and held inside the micro channel for inhibiting the fouling. The experiment performed with the same parameter group used in Comsol simulation and results shared which are similar to the simulation results. 3.2 µm particles (Red fluorescent) are separated from the 9.8 µm particles (Green fluorescent) in both simulation and experiment. This work was supported by TUBITAK under Project No. 111M730.