Yüksek LisansAçık Erişim

Ön arıtılmış deponi sızıntı suyunda mikroalg büyütülmesi

2015
0 görüntülenme
0 i̇ndirme
Danışman: Yrd. Doç. Dr. Mahmut Altınbaş

Özet (EN)

79.7% of world's energy needs is supplied by fossil fuels. Estimates indicate that oil will run out in the next 35 years, natural gas will run out in the next 37 years, and coal will run out in the next 107 years. Renewable and environmentally friendly energy resources should developed to address the world's energy deficit. Biodiesel is a promising energy source that provides this necessary. Although Biodiesel is a renewable and environmentally friendly energy source, because of the production of biodiesel, which is alternative the petro-diesel usage, is more expensive than production petro-diesel, it does not have widespread use. Many different methods have been proposed in order to reduce the cost of biodiesel. One of these systems is biorafinery system. When carrying out the production of biodiesel by using biorafinery system, while also valuable products/output work is performed. On the other hand, to sustain ecological balance to protect our lifestyle and future of our offspings waste reduction and recycling must carried out even more efficiently than before. Lately, In line with this vision, nitrogen and phosphorus, which before given the receiving environment with the effluent water, intended to benefit. In this study, algal strain Synechocystis sp. PCC 6803 was growth on pre-treated landfill leachate (PLL) with different dilution rate and determining the biodiesel production potential and wastewater cleaning efficiency of nitrogen and phosphorus were measured. Our goal is to determine that using leachate as medium cost-effective solution for algal biodiesel production and Synechocystis sp. PCC 6803 is usable for the nitrogen and phosphorus removal from pre-treated landfill leachate. Synechocystis sp. PCC 6803 strain grown on mediums, of which dilution rate differ from each other, for 500 hours. These dilutions are 20%, 40%, 60%, 80% and 100%. However, Synechocystis sp. PCC 6803 not grown on medium contains 100% of pre-treated landfill leachate. Thus, data of these mediums were not been taken into account. Our strain were cultured at 32±2°C with a continuous white led illumination with intensity of 100-300 µE/m–2*s-1. Our cultures were aerated with air that aeration speed is 1 L/min. Growths of the culture was determined via optical density measurement. For each different medium, which has different dilution rate, optical density time graphs was prepared. According to our data, with increased concentration of pre-treated landfill leachate organic N removal efficeny dropped. Especially, medium %80 PLL, removal efficiency dropped significantly. Removal efficiency of %20 PLL, %40 PLL, %60 PLL and %80 PLL are about %90, %86, %83 and %41, respectively. According to our data, with increased concentration of pre-treated landfill leachate TKN removal efficeny dropped. Especially, medium %80 PLL, removal efficiency dropped significantly. Removal efficeny of %20 PLL, %40 PLL, %60 PLL and %80 PLL are about %51, %44, %40 and %20, respectively. According to our data, with increased concentration of pre-treated landfill leachate ammonia-N removal efficeny was drop. However, ther is no quick drop observed from grap. Removal efficeny of %20 PLL, %40 PLL, %60 PLL and %80 PLL are about %28, %19, %14 and %7, respectively. According to our data, with increased concentration of pre-treated landfill leachate organic N removal efficeny was drop. Especially, medium %80 PLL, removal efficeny drop significanly. Removal efficeny of %20 PLL, %40 PLL, %60 PLL and %80 PLL are about %90, %86, %83 and %41, respectively. Removal efficeny of orthophosphate of medium %20 PLL, %40 PLL, %60 PLL and %80 PLL are about %94, %96, %89 and %88, respectively according to our work. Maximum OD680 value which was seen is 1,816 for medium with 20% dilution rate. Spesific growth rate of medium with 20% dilution was found 0,00753 h-1 and doubling time of medium with 20% dilution was determined 91,96 hours. Maximum OD680 value which was seen is 1,089 for medium with 40% dilution rate. Spesific growth rate of medium with 40% dilution was found 0,00887 h-1 and doubling time of medium with 40% dilution was determined 78,35 hours. Maximum OD680 value which was seen is 0,815 for medium with 60% dilution rate. Spesific growth rate of medium with 60% dilution was found 0,00937 h-1 and doubling time of medium with 60% dilution was determined 73,95 hours. Maximum OD680 value which was seen is 0,578 for medium with 80% dilution rate. Spesific growth rate of medium with 80% dilution was found 0,01409 h-1 and doubling time of medium with 80% dilution was determined 49,20 hours. According to our results, high orthophosphorus removal rate was observed. This is expected results because, our medium contain very low orthophosphorus concentration. Because of the low growth amount im medium, total kjeldahl nitrogen removal efficieny is not high.It thought that high organic N removal amount caused by contamination of heterotrophic organisms.

Yazar

Dr. Umut Metin

Bu Yayına Nasıl Atıf Yapılır

Umut Metin (Master Thesis). Ön arıtılmış deponi sızıntı suyunda mikroalg büyütülmesi, 2015, Istanbul Technical University.

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