Isolation and identification thermophilic bacteria from Güçlükonak and Dargeçit hot springs

In Dargeçit, Mardin and in Güçlükonak, Sirnak districts of hot springs waterand mud samples of bacteria were isolated. Morphological, physiological,biochemical characteristics, lipid analysis and identification 16S rRNA sequenceanalysis of these bacteria was performed and identified.Two strains isolated from hot springs of Hısta (Güçlükonak) was called GE1 andGE2. These isolates were rod shaped, gram-positive, aerobic, and sports animatedcharacter has been identified as forming thermophile. Bacillus of GE1 is usuallyfound as bilateral. Otherwise, bacillus of GE2 is found as asingle. 30-65 ºC (optimum60 °C) for GE1 and 35-65 ºC (optimum 60 °C) for GE2 was found as thetemperature range of reproductive. Optimum growth pH for GE1 and GE2 werefound 9 and 9,5. Growt graphic of GE1 and GE2 were drawn and produce thehighest rate of they were seen in the fifteenth and twelfth hours, respectively. Starch,catalase, urease, phosphatase tests of GE1 and GE2 have determined positive. 1%NaCI tolerance of isolates were found to show. GE1 used galactose, sucrose, maltoseand lactose as carbon source; glucose, fructose and glycerol which are used in a waythat very little was stated. GE2 used maltose as the carbon source, glucose and wereused as the weak.Strains isolated from hot springs of Ilısu (Dargeçit) was named AH1 andAH2. These bacteria are rod-shaped, gram-positive, spore forming, aerobic, activethermophilic properties. Cells of these bacteria are usually bilateral. 30-65 º C(optimum 60 ° C) was found as temperature range of reproductive for AH1, 30-65(optimum 65 ° C) was found as the temperature range of reproductive for AH2.Reproductive pH range was found 5.5-10 (optimum 7.5) for AH1. reproductive pHrange 6-11 (optimum 7.5) was found. Reproductive pH range was found 6-11(optimum 7.5) for AH2. The optimum time to produce for AH1 and AH2 have beenseen 24th and 15th hours, respectively. Starch hydrolysis, catalase, casein, lipase andphosphatase tests of isolates were positive. the lysozyme sensitivity and sodiumazide sensitivity of both isolates have been seen positive. 2 % NaCI tolerance ofisolates were found to show. AH1 as a carbon source used galactose and glycerolpoorly. AH2 also used sucrose, lactose, and glycerol in a weak manner weredetermined.Lipid analysis of GE1, GE2, AH1, the comparison with standard isolatesAlicyclobacillus acidocaldarius subsp. rittmannii and Geobacillus toebii,phospholipids, glycolipids and amino lipids of isolates were similar to the standard ofthe Geobacillus toebii.16S rRNA gene sequence database of GE1 by comparing with other speciesin the genus Geobacillus species were found to sympathize with. A new species ofthe genus Geobacillus is thought to be.16S rRNA gene of GE2 compared with other types of analysis is showedGeobacillus kaue'a sympathize.16S rRNA gene sequence analysis of AH1 and AH2 in comparison with theexisting data base is similar to Anoxybacillus genus and Anoxybacillus flavithermuswas 99 % affinity.Keywords: Bacterial identification, thermophilic bacteria, 16S rRNA, Hot springs