Effects of ozonated oils (sesame oil, nigella sativa oil and hypericum perforatum oil) on wound healing process in rats

In this study, the effects of three different ozonated oils (sesame oil, Nigella sativa oil and Hypericum perforatum oil) on wound closure rate, healing process and possible complications were examined macroscopically and microscopically. Twenty-one adult Wistar albino female rats were used in the study. Subjects were divided into three groups, i.e. (Early wound healing (7 days), medium wound healing (14 days) and late wound healing (21 days). Four full-thickness skin wounds of equal size (10 mm in diameter) to the back regions of all rats were formed. The wound was left open during healing. While the first wound (control group) received no treatment the second wound, ozonated sesame oil, in the third wound ozonated Nigella sativa oil and lastly ozonated hypericum perforatum oil were used. The first, second and third main groups were euthanized on days 7, 14 and 21, respectively. There was no significant difference in wound healing between groups in the first 7-day. In the 14 day groups, it was found that the healing was better in the group of Nigella sativa oil and sesame oil group (P<0.05). In 21 day groups all wound healing was improved, but Nigella sativa oil group had earlier improvement compared to the others (P<0.01).As a result, the best wound healing was achieved with Nigella sativa oil and sesame oil. Keywords: Wound healing, ozonated Sesame oil, ozonated Nigella sativa oil, ozonated Hypericum perforatum oil