Kurt üzümü polisakkaritlerinin, overektomili sıçanların anksiyete, depresyon ve öğrenme davranışları üzerine etkileri

To investigate the effects of the Lycium barbarum L. polysaccharides (LBP) on anxiety, depression and learning behaviors in ovariectomized female rats using the open field, elevated plus maze, Porsolt and Morris water maze tests. Two weeks after ovariectomy operations, rats were divided into seven major groups: control [distile water, 3 mL/kg, oral gavage (o.g), per day], low dose of LBP (20 mg/kg, 3 mL/kg, o.g., per day), high dose of LBP (200 mg/kg, 3 mL/kg, o.g., per day), 17 beta estradiol (1 mg/kg, 3 mL/kg, o.g., per day), diazepam (1 mg/kg, 3 mL/kg, o.g., per day), imipramine (2,5 mg/kg, 3 mL/kg, o.g., per day), donepezil (1 mg/kg, 3 mL/kg, o.g, per day) and two minor group within the each major group: pseudo ovariectomized rat (SHAM) and overiectomized (OVX) rat groups. The treatments were applied for 30 consecutive days and then serum and brain tissue sample of all rats were collected. Biochemical [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), malondialdehyde (MDA) and 17 beta estradiol (17β-ES)] and immunohistochemical [brain drived neutrophic factor (BDNF), serotonin receptor (SER) and apoptosis] analysis of the samples were performed. The results of the behavioral experiments demonstrated that the high dose and low dose of LBP administrated rats less anxious, less depressive and more memory performances than control groups. Biochemical study findings showed that serum of the high dose of LBP administrated rats showed high level SOD, low level of MDA. Immunohistochemical study findings demonstrated that high dose of LBP and drugs administrated groups had high level of serotonin receptor and BDNF positive cell and, low level of TUNEL positive cell. In conclusion, high dose of LBP treatments decrease anxiety and depression-like behaviors and increase learning and memory performance by increasing antioxidant enzyme activities and hippocampal SER, and BDNF neurotransmitter levels and decreasing TUNEL positive cell count of ovariectomized female rats.