Effect of estrogene and progesterone through mitogen activated protein kinase in the endometrium cells

During menstrual cycle endometrium makes serial changes in response to steroid hormones for a succesful implantation. Coordinated production of these hormones takes a key role in this change that happen in a cyclic way. Estrogen is responsible for endometrial proliferation in the first phase of cycle. Progesteron differentiates the tissue by suppressing the increased estrogen effect. Fulvestrant, estrogen receptor antagonist, binds and inhibits estrogen receptor and proliferation is inhibitedIn our study in Ishikawa endometrium epithelium cells the effect of 17-ß-östradiol, fulvestrant and progesteron on proliferation is evaluated with comparison of S phase cell rates using 5-bromo 2?-deoxy-uridin labeling technique. Mitogen activated protein kinases (MAPK) are responsible from progress of functions such as embryogenesis, proliferation, differentiation and apoptosis and respond to stress. Activation levels of P38, JNK and c-jun which is immediately early response protooncogene are the member of MAPK family were examined under the influence of steroid hormones. In the cells which incubated with 17-ß-östradiol, S phase cell rates increased statistically but fulvestrant did not antagonized proliferative effect of estrogen. On the cells that progesteron is applied proliferation rate did not change statistically. During time and concentration that is applied, estrogen fulvestrant, and progesteron did not change the expression levels of JNK, phospho p38 and phospho c-junAccording to the findings that obtained, we conclude that in endometrium epithelial cells, estrogen and progesteron that control cycle phases show their effect independently from expressions of JNK, phospho p38 and phospho c-jun.