Investigation of mycoplasma bovis in bovine lung samples with pneumonia by real time PCR

The aim of this study was to investigate Mycoplasma (M.) bovis from bovine lungs with pneumonia by Real-Time PCR. M. bovis was identified in 52 out of 100 bovine lung samples diagnosed with pneumonia macroscopically by Real-Time PCR wherease the presence of various bacterial agents was determined in 58 of the samples by conventional bacteriological methods. In the study, Escherichia coli was detected in the highest rate (25%) after M. bovis while Pasteurella multocida, Klebsiella pneumoniae, Sphingomonas paucimobilis, Mannheimia haemolytica, Staphylococcus epidermidis and Staphylococcus spp. were isolated from 13%, 7%, 7%, 4%, 1% and 1% of the samples, respectively. Also, bacterial agent was not isolated from 42 of lung samples by conventional bacteriological methods. The findings of the study indicated that M. bovis was an important causative agent of respiratory diseases in cattle, and that the presence of other bacterial agents should be considered in the diagnosis and treatment of the disease. Keywords: Bovine, Lung, Mycoplasma bovis, Real Time-PCR