Stevıa[stevıa rebaudıana (bert.)] bitkisinin ın vıtro çoğaltımı ve Türkiye'de ticari olarak yetiştirilebilecek yüksek stevıol glycosıde içeren çeşit geliştirilmesi

This study involves two main parts; in vitro propagation and field cultivation. In vitro propagation studies were conducted at the Biotechnology Laboratory of Abant İzzet Baysal University, Department of Biology, Bolu and cultivation experiments were conducted at the Research field of Polisan Kimya A.Ş. in Yalova and Balıkesir and private farm in Adana owned by Tevfik Ramazanoğlu during two successive plantation periods. In all of our experiments, plant height, crop dry leaf yield and stevioside contents were studied. The experiments were planted according to randomized block design in factorial restriction (RBD) with four replications. At laboratory stage; propagation of stevia via conventional and tissue culture techniques were compared. The seeds of two stevia genotypes (GF and CB) were germinated in vitro under different light regimes ranging from 1000 to 5000 lux. Higher germination rates were achieved between 2000-3000 lux with a frequency of 50% germination within two days. According to the results, the highest plant height and leaf yield were determined in Adana at 2014 spring plantation. The highest stevioside and RebA ratios in dry leaves were obtained in Yalova region at 2nd harvest.